| 张国建 | 博士 | 教授 | 博士生导师 |
科 室： | 天然药物化学研究室 | |||
办公电话： | 0532-82032971 | 电子邮箱： | zhangguojian@ouc.edu.cn | |
联系地址： | 山东省青岛市鱼山路5号中国海洋大学医药学院海洋馆205 邮编：266003 | |||
研究方向： | 1.海洋微生物活性天然产物分子的发现 2. 微生物生物合成和代谢工程研究 | |||
个人简介 |
|
|
|
|
山东省泰山学者青年专家（2020），中国海洋大学“青年英才工程”第一层次教授。2006年6月毕业于中国海洋大学药学专业，获得学士学位；2011年6月毕业于中国海洋大学药物化学专业，获得博士学位。2012年1月至2017年9月先后在纽约州立大学布法罗分校（Blaine Pfeifer 课题组）和俄克拉荷马大学（Robert Cichewicz课题组）从事博士后研究。在Sci. Adv., Curr. Opin. Biotech., J. Nat. Prod., Biotechnol. Bioeng.等期刊发表SCI收录论文30余篇。主持国家自然科学基金面上（41976105），参与国家自然科学基金重大项目（81991522）、国家重点研发项目（2019YFC0312500）等课题。担任Curr. Opin. Biotech.,Fermentation等杂志客座编辑和中国抗生素杂志青年编委，并担任ACS Synth. Biol., J. Nat. Prod., App. Microbial. Biotechnol.等二十余个期刊审稿工作。主要研究方向包括：海洋环境来源微生物功能基因和代谢产物的潜能挖掘、活性次级代谢产物的发现；运用代谢工程和合成生物学方法解析和改造生物合成途径，基于途径重构和工程化菌株获得结构新颖的活性分子。 | ||||
教育背景 |
|
|
|
|
2006.09~2011.07 | 中国海洋大学 | 药物化学 | 博士 | |
2002.09~2006.06 | 中国海洋大学 | 生物技术 | 学士 | |
工作经历 |
|
|
|
|
2022.12~至今 | 中国海洋大学 医药学院 | 教授 |
| |
2017.07~2022.12 | 中国海洋大学 医药学院 | 副教授 |
| |
2014.12~2016.12 | 俄克拉荷马大学 | 博士后 |
| |
2012.01~2014.12 | 纽约州立大学布法罗分校 | 博士后 |
| |
学术兼职 |
|
|
|
|
Curr. Opin. Biotech., Fermentation等杂志客座编辑和中国抗生素杂志青年编委，并担任ACS Synth. Biol., J. Nat. Prod., App. Microbial. Biotechnol.等三十余个期刊审稿工作。 | ||||
荣誉奖励 |
|
|
|
|
山东省泰山学者青年专家（2020） | ||||
承担课程 |
|
|
|
|
药学分子生物学（本科生课程） 基因工程技术 （本科生国际课程） 海洋微生物药物（研究生课程） 高等分子生物学（研究生课程） Marine Natural Products（国际研究生课程） | ||||
研究进展 |
|
|
|
|
① 基于基因组分析采掘，结合现代天然产物化学研究方法开发海洋微生物代谢潜能，获得了联苯杂环类、特拉姆酸类、混源萜以及寡肽类等多种结构新颖的天然产物。上述结构在抗肿瘤、抗炎、抗感染等领域表现出了良好的应用潜力。
②利用代谢工程、组合生物合成和异源表达技术改造和优化天然产物生物合成途径，获得工程化菌株和结构新颖的天然产物分子。前期利用大肠杆菌宿主成功完成了红霉素、水杨酸糖苷衍生物等系列药物分子的异源表达。目前开展针对于莽草酸途径的代谢工程研究以及萜类（alternarenes类混源萜）、多肽（peptaibols类抗菌肽）和二酮哌嗪（penicisulfuranol类多硫代二酮哌嗪）等活性分子的生物合成研究。
| ||||
| ||||
代表性成果 | ||||
代表性论文 |
|
|
|
|
Guowei Liu, Dan Liu, Zongyuan Li, Jieying Jiao, Xuewen Hou, Xiaomin Zhang, Qian Che, Tianjiao Zhu, Dehai Li, Guojian Zhang*. Overexpression oftranscriptional regulator andtailoring enzyme leads to thediscovery of anti-inflammatorymeroterpenoids from marinederivedfungus Alternariaalternata JJY-32.Fron. Mar. Sci., 2022, DOI: 10.3389/fmars.2022.1015684. Xuewen Hou, Changlong Li, Runfang Zhang, Yinping Li, Huadong Li, Yundong Zhang, Han-Shen Tae, Rilei Yu, Qian Che, Tianjiao Zhu, Dehai Li*, Guojian Zhang*. Unusual Tetrahydropyridoindole-Containing Tetrapeptideswith Human Nicotinic Acetylcholine Receptors TargetingActivity Discovered from Antarctica-Derived Psychrophilic. Mar. Drugs, 2022, 20, 593. Kaijin Zhang#, Guojian Zhang#, Xuewen Hou, Chuanteng Ma, Junyu Liu, Qian Che, Tianjiao Zhu, Dehai Li*. A Fungal Promiscuous UbiAPrenyltransferase Expands theStructural Diversity of Chrodrimanin-Type Meroterpenoids. Org. Lett.,2022, 24, 2025−2029. Xuewen Hou, Ruonan Sun, Yanyan Feng, Runfang Zhang, Tianjiao Zhu, Qian Che, Guojian Zhang*, Dehai Li*. Peptaibols: diversity, bioactivity, and biosynthesis. Eng. Microbiol., 2022, 2, 100026. Zilu Tang, Yimin Chang, Tianjiao Zhu, Qian Che, Dehai Li*, Guojian Zhang*. Antibacterial angucyclinone and α-pyrone derivatives from desert-derived Nocardiopsisdassonvillei HDN 154151. J. Antibiot., 2022, 75, 380-384. Guoliang Zhou, Tianjiao Zhu, Qian Che, Guojian Zhang*, Dehai Li*. Structural diversity and biological activity of natural p-terphenyls. MLST., 2022, 4, 62-73. Yimin Chang, Qian Che, Li Xing,Chuanteng Ma,Yaxin Han,TianjiaoZhu, Blaine A. Pfeifer, Jixing Peng, Guojian Zhang* and Dehai Li*. Antibacterial p-Terphenyls with rare 2,2'-bithiazole moiety isolated from marine-derived actinomycete Nocardiopsis sp. HDN154086. J. Nat. Prod.2021, 84, 4, 1226–1231. Guoliang Zhou, Tianjiao Zhu, Qian Che, Guojian Zhang*, Dehai Li*. Structural diversity and biological activity of natural p-terphenyls. Marine Life Science & Technology2021, DOI:10.1007/s42995-021-00117-8. Chuanteng Ma, Kaijin Zhang, Xianyan Zhang, Guowei Liu, Tianjiao Zhu, Qian Che, Dehai Li and Guojian Zhang*. Heterologous expression and metabolic engineering tools for improving terpenoids production. Curr. Opin. Biotech.,2021, 69, 281-289. Ruonan Sun, Yanyan Feng, Hengyi Xu, Xuewen Hou, Tianjiao Zhu, Qian Che, Blaine Pfeifer, Guojian Zhang* and Dehai Li*. An Efficient Marker Recycling System for Sequential Gene Deletion in a Deep Sea-derived Fungus Acremonium sp.HDN16-126. Synth. Syst. Biotechnol.,2021, 6, 2, 127-133. Lu Wang, Xianyan Zhang, Kaijin Zhang, Xiaomin Zhang, Tianjiao Zhu, Qian Che, Guojian Zhang* and Dehai Li*. Overexpression of Global RegulatorbrlaeA Leads to the Discovery of New Polyketide in Fungus Penicilliumrocae HDN-12-143. Front. Chem., 2020, online. DIO: 89/fchem.2020.00270. Lu Wang, Jieying Jiao, Dan Liu, Xiaomin Zhang, Jing Li, Qian Che, Tianjiao Zhu, Dehai Li* and Guojian Zhang*. Cytotoxic Meroterpenoids from the Sponge‐Associated Fungus Alternaria sp. JJY‐32. Chem. Biodivers., 2020, 17, e2000226. Front. Xueping Ge, Chunxiao Sun, Yanyan Feng, Lingzhi Wang, Jixing Peng, Qian Che, Qianquan Gu, Tianjiao Zhu, Dehai Li and Guojian Zhang*. Anthraquinone Derivatives from a Marine-Derived Fungus Sporendonemacasei HDN16-802. Mar. Drugs, 2019, 17:334. Tingting Zhao, Yimin Chang, Tianjiao Zhu, Jing Li, Qianqun Gu, Dehai Li, Qian Che* and Guojian Zhang*. α-Pyrone derivatives with cyto-protective activity from two TaklaMakan Desert Soil Derived Actinomycete Nocardiopsis Strains Recovered in Seawater Based Medium.Nat. Prod. Res., 2019, 33(17), 2498-2506. Chunxiao Sun, Shah Mudassir, Zhenzhen Zhang, Yanyan Feng, Yimin Chang, Qian Che, Qianqun Gu, Tianjiao Zhu, Guojian Zhang* and Dehai Li*. Secondary Metabolites from Deep-Sea Derived Microorganism. Curr. Med. Chem. 2019, DIO: 10.2174/0929867326666190618153950. Guihong Yu, Guangwei Wu, Zichao Sun, Xiaomin Zhang, Qian Che, Qianqun Gu, Tianjiao Zhu, Dehai Li and Guojian Zhang*. Cytotoxic tetrahydroxanthone dimers from the mangrove-associated fungus Aspergillus versicolor HDN1009, Mar. Drugs, 2018, 16:335. Lei Fang#, Guojian Zhang#*, Omar El-Halfawy, Eric D. Brown and Blaine. A. Pfeifer*. Broadened Glycosylation Patterning of Heterologously Produced Erythromycin. Biotechnol. Bioeng., 2018, 115, 2771-2777. Zhenzhen Zhang, Xueqian He, Guangwei Wu, Congcong Liu, Changjun Lu, Qianqun Gu, Qian Che, Tianjiao Zhu, GuojianZhang,* and Dehai Li*. Aniline-tetramic Acids from the Deep-sea Derived Fungus Cladosporium sphaerospermum L3P3 Cultured with the HDAC Inhibitor SAHA. J. Nat. Prod. 2018, 81 (7), 1651–1657. Ruiquan Qi, Blaine A Pfeifer* and Guojian Zhang*, Engineering heterologous production of salicylate glucoside and glycosylated variants.Front. Microbiol., 2018, 9, 2441. Zhenzhen Zhang, Xueqian He, Qian Che,Guojian Zhang*, Tianjiao Zhu, Qianqun Gu and Dehai Li*. Sorbicillasins A-B and Scirpyrone K from a Deep-Sea-Derived Fungus, Phialocephala sp. FL30r. Mar. Drugs, 2018, 16 :245. Charles H Jones#, Guojian Zhang#, RoozbehNayerhoda, Marie Beitelshees, Andrew Hill, Pooya Rostami, Yi Li, Bruce A Davidson, Paul Knight, Blaine A Pfeifer. Comprehensive vaccine design for commensal disease progression. Sci. Adv., 2017, 3, e1701797. (#Co-first author). Guojian Zhang, Jing Li, Tianjiao Zhu, Qianqun Gu and Dehai Li. Advanced tools in marine natural drug discovery. Curr. Opin. Biotech. 2016, 43, 13-23. Guojian Zhang, Yi Li, Lei Fang and Blaine A. Pfeifer. Tailoring Pathway Modularity in the Biosynthesis of Erythromycin Analogues Heterologously Engineered in E. coli.Sci. Adv., 2015, 1, e1500077. Guojian Zhang, Guangwei Wu, Tianjiao Zhu, Tibor Kurtán, Attila Mándi, Jieying Jiao, Jing Li, Xin Qi, Qianqun Gu, and Dehai Li. Meroterpenoids with Diverse Ring Systems from the Sponge-Associated Fungus Alternaria sp. JJY-32. J. Nat. Prod., 2013, 76, 1946–1957. Xinying Yang#, Guojian Zhang#, Xuelian Tang, Jieying Jiao, Sung Yeon Kim, Joo Young Lee, Tianjiao Zhu, Dehai Li, Yong Gab Yun and Qianqun Gu. Toll-like Receptor 4/Nuclear Factor-κB Signaling Pathway is Involved in ACTG-toxin H-mediated Anti-inflammatory Effect. Mol. Cell. Biochem., 2013, 374, 29-36. (#Co-first author). Guojian Zhang, Shiwei Sun, Tianjiao Zhu, Zhenjian Lin, Jingyan Gu, Dehai Li and Qian-qun Gu. Antiviral Isoindolone Derivatives from an Endophytic Fungus Emericella sp. Associated with Aegicerascorniculatum.Phytochemistry,2011, 72, 1436-1442. | ||||
出版著作 |
|
|
|
|
Ruiquan Qi, Guojian Zhang, B.A. Pfeifer. In Engineering Escherichia coli for bacterial natural product production. Chaitan Khosla, Sean Brady, Jay Keasling, Eds; Comprehensive Natural Products III: Chemistry and Biology. Elsevier, 2019. Lei Fang, Guojian Zhang, Blaine A Pfeifer. In Engineering of E. coli for Heterologous Expression of Secondary Metabolite Biosynthesis Pathways Recovered from Metagenomics Libraries; Trevor C. Charles, Mark R. Liles, Angela Sessitsch, Eds; Functional Metagenomics: Tools and Applications. Springer-Verlag London Ltd, 2017. Guojian Zhang and Blaine A. Pfeifer. In Production of Therapeutic Products; Anne Osbourn, Rebecca Goss, Guy T. Carter, Eds; Natural Products: Discourse, Diversity, and Design; Wiley-Blackwell Ltd, 2014. Yi Li, Guojian Zhang, Blaine A. Pfeifer. In Current and Emerging Options for Taxol Production; Jens Schrader, Jörg Bohlmann, Eds; Biotechnology of Isoprenoids. Springer-Verlag London Ltd, 2014. | ||||
专利 |
|
|
|
|
| ||||
项目课题 | ||||
2020.01-2023.12 国家自然科学基金面上项目（41976105），主持，74.4万元。 2021.01-2025.12 山东省泰山学者青年专家项目（tsqn202103153），主持50.0万元 2020.01-2024.12国家自然科学基金重大项目（81991522），参与，50.0万元 2019.08-2021.12 国家重点研发项目（2019YFC0312500），参与，主持子任务，110.0万元 2017.07-2022.07中国海洋大学青年英才工程项目启动基金，主持，15.0万元 2019.06-2021.06 海洋科学与技术国家试点实验室开放基金，参与，7.5万元
| ||||
